List of Publications

 

Le, T.T., Duren, H.M., Sllpchenko, M.N., Hu, C.D., and Cheng, J.X. Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans. In press (2009)

 

Hiatt, S.M., Duren, H.M. Shyu, Y., Ellis, R.E., Hisamoto, N., Matsumoto, K., Kariya, K., Kerppola, T.K., and Hu, C.D. C. elegans FOS-1 and JUN-1 regulate plc-1 expression to control ovulation. Mol. Biol. Cell 20:3888-3895 (2009)

 

Yuan, Z., Gong, S., Song, B., Mei, Y., Hu, C., Li, D., Thiel, G., Hu, C.D., and Li, M. Opposing role for ATF2 and c-Fos in c-Jun-mediated apoptosis induced by potassium deprivation in cerebellar granule neurons. Mol. Cell. Biol. 29:2431-2442 (2009)

 

Xu, Y., Yang, W.H., Gerin, I., Hu, C.D., Hammer, G.D., and Koenig, R.J. DAX-1 and steroid receptor RNA activator (SRA) function as transcriptional coactivators for steroidogenic factor-1 in steroidogenesis. Mol. Cell. Biol. 29:1719-1734 (2009)

 

Deng, X., Liu, H., Huang, J., Cheng, L., Keller, E.T., Parsons, S.J., and Hu, C.D. Ionizing radiation induces prostate cancer neuroendocrine differentiation through interplay of CREB and ATF2: Implications for disease progression. Cancer Res. 68:9663-9670 (2008)

 

Shyu, Y. and Hu, C.D. Fluorescence complementation: an emerging tool for biological research. Trends Biotechnol. 26:622-630 (2008)

 

Shyu, Y., Suarez, C., and Hu, C.D. Visualization of ternary complexes in living cells by using a BiFC-based FRET analysis. Nat. Protoc. 3:1693-1702 (2008)

 

Vidi, P.A., Chemel, B.R., Hu, C.D., Watts, V.J. Ligand-Dependant Oligomerization of Dopamine D2 and Adenosine A2A Receptors in Living Neuronal Cells. Mol. Pharmacol. 74:544-551 (2008)

 

Hiatt, S.M., Shyu, Y., Duren, H.M, and Hu, C.D. Bimolecular fluorescence complementation (BiFC) analysis of protein interactions in living C. elegans. Methods, 45:185-191 (2008)

 

Shyu, Y.,  Fox, SM., Duren, HM., Ellis, R.E., Kerppola, T.K. and Hu, C.-D. Visualization of protein interaction in living Caenorhabditis elegans using bimolecular fluorescence complementation (BiFC) analysis. Nat. Protoc., 4:588-596 (2008)

 

Shyu, Y., Suarez, C., and Hu, C.-D. Visualization of AP-1-NF-kB ternary complexes in living cells by using a BiFC-based FRET. Proc. Natl. Acad. Sci. U.S.A., 105:151-156 (2008)

 

Shyu, Y. Akasaka, K., and Hu, C.D. Bimolecular fluorescence complementation (BiFC): A colorful future in drug discovery. Sterling-Hoffman Life Science Journal, July 2007. http://www.sterlinglifesciences.com/newsletter/articles/article006.html

 

Tong, E.H.Y., Guo, J.J., Haung, A., Liu, H., Hu, C.D., Chung, S.S.M., and Ko, C.B. Regulation of nucleocytoplasmic trafficking of transcription factor OREBP/TonEBP/NFAT5. J. Biol. Chem. 281:23870-23879 (2006)

 

Liu, H., Deng, X., Shyu, Y., Li, J.J., Taparowsky, EJ., and Hu, C.-D. Mutual regulation of c-Jun and ATF2 by transcriptional activation and subcellular localization. The EMBO J., 25:1058-1069 (2006).

 

Wang ,KZQ, Wara-Asparati, N., Boch, J.A., Yoshida, Y., Hu, C.-D., Galson, D.L., and Auron, P.E. TRAF6 activation of PI3 kinase-dependent cytoskeletal changes is cooperative with Ras and mediated by an interaction with cytoplasmic c-Src. J. Cell Sci., 119, 1579-1591 (2006).

 

Shyu, Y., Liu, H., Deng, X., and Hu, C.-D. Identification of new fluorescent fragments for BiFC analysis under physiological conditions. BioTechniques, 40:61-66 (2006).

 

Hu, C.-D., Grinberg, A.V. and Kerppola, T.K. Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation (BiFC) Analysis. (ed. Coligan JE,  Dunn BM, Speicher DW, Wingfield PT) Curr. Protoc. Protein Sci. 41:19.10.1-19.10.21. Hoboken, John Willey & Sons, 2005.

 

Hu, C-D., Grinberg A., and Kerppola T. Visualization of protein interaction in living cells using bimolecular fluorescence complementation (BiFC) analysis. Current Protocol in Cell Biology. (ed. Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwartz J, Yamada KM), Hoboken: John Wiley & Sons, pp21.3.1-21.3.21, 2005.

 

Hu, C-D. and Kerppola TK. Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis. Protein-Protein Interactions (ed. P. Adams and E. Golemis), Cold Spring Harbor Laboratory Press, pp673-693, 2005.

 

Grinberg A, Hu, C.-D., and Kerppola T. Visualization of Myc/Max/Mad family dimers and the competition for dimerization in living cells. Mol. Cell. Biol. 24, 4294-4308 (2004).

 

Hu, C.-D. and Kerppola, T. Simultaneous visualization of interactions between multiple proteins in living cells using multicolor bimolecular fluorescence complementation analysis. Nat. Biotechnol. 21, 539-545 (2003).

 

Hu, C.-D. Chinenov, Y., and Kerppola, T Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol. Cell. 9, 789-798 (2002).

 

Gao X., Satoh T., Liao Y., Song C., Hu C.-D., Kariya K., and Kataoka T. Identification and characterization of RA-GEF-2, a Rap guanine nucleotide exchange factor that serves as a downstream target of M-Ras. J. Biol. Chem. 276, 42219-42225 (2001).

 

Jin T.-G., Satoh T., Liao Y., Song C., Gao X., Kariya K., Hu C.-D., and Kataoka T. Role of the CDC25 homology domain of phospholipase C-epsilon in amplification of Rap1-dependent signaling. J. Biol. Chem. 276, 30301-30307 (2001).

 

Liao, Y., Satoh, T., Gao, X., Jin, T.-G., Hu, C.-D., and Kataoka, T. RA-GEF-1, a guanine nucleotide exchange factor for Rap1, is activated by translocation induced by association with Rap1GTP and enhances Rap1-dependent B-Raf activation. J. Biol. Chem. 276, 28478-28483 (2001).

 

Song*, C., Hu*, C.-D., Masago, M., Kariya, K., Yamawaki-Katatoka, Y., Shibatohge, M., Sen, H., Wu, D., Satoh, T., and Kataoka, T. Regulation of a novel human phospholipase C, PLC-e through differential membrane targeting by Ras and Rap1. J. Biol. Chem. 276, 2752-2757 (2001).                     *Equal contribution to this work

 

Sen, H., Hu, C.-D., Wu, D., Song, C., Yamawaki-Katatoka, Kotani, J., Okada, T., Shima, F., Kariya, K., and Kataoka, T. Role of Raf-1 conserved region 2 in regulation of Ras-dependent Raf-1 activation. Biochem. Biophys. Res. Commun., 271, 596-602 (2000).

 

Shima, F., Okada, T., Kido, M., Sen, H., Tanaka, Y., Tamada, M., Hu, C.-D., Yamawaki-Kataoka, Y., Kariya, K., and Kataoka, T. Association with CAP forms a second Ras-binding site of yeast adenylyl cyclase which mediates activation by posttranslationally modified Ras protein. Mol. Cell. Biol. 20, 26-33 (2000).

 

Liao, Y., Kariya, K., Hu, C.-D., Shibatohge, M., Goshima, M., Okada, T., Watari, Y., Gao, X., Jin, T.-G., Yamawaki-Katatoka, Y., and Kataoka, T. RA-GEF, a novel Rap1A guanine nucleotide exchange factor containing a Ras/Rap1A-associating domain, is conserved between nematode and humans. J. Biol. Chem. 274, 37815-37820 (1999).

 

Tanaka, Y., Minami, Y., Mine, S., Hirano, H., Hu, C.-D., Fujimoto, H., Fujii, K., Saito, K., Tsukada, J., van Kooyk, Y., Figdor, C. G., Kataoka, T., and Eto, S. H-Ras signals to cytoskeletal machinery in induction of integrin-mediated adhesion of T cells. J. Immunol., 163, 6209-6216 (1999).

 

Okada, T., Hu, C.-D., Jin T.-G., Kariya, K., Yamawaki-Katatoka, Y., and Kataoka, T. The strength of interaction at the Raf cysteine-rich region domain is a critical determinant of response of Raf to Ras family small GTPase. Mol. Cell. Biol. 19:6057-6064 (1999).

 

Hu, C.-D., Kariya, K., Okada, T., Qi, X., Song, C., and Kataoka, T. Effect of phosphorylation on activities of Rap1A to interact with Raf-1 and to suppress Ras-dependent Raf-1 activation , J. Biol. Chem., 274, 48-51 (1999).

 

Watari, Y., Kariya, K., Shibatohge, M., Liao, Y., Hu, C.-D., Goshima, M., Tamada, M., Kikuchi, A., and Kataoka, T. Identification of Ce-AF-6, a novel Caenorhabditis elegans protein, as a putative Ras effector, Gene, 224, 53-58 (1998).

 

Kataoka, T., Kariya, K., Yamawaki-Kataoka, Y., Hu, C.-D., Shirouzu, M., Yokoyama, S., Okada, T., and Shima, F. Isoprenylation-dependent and independent interaction of Ras with its effectors.  In Kuzumaki, N. Cytoskeleton and G-Protein in the Regulation of Cancer. Hokaido University Medical Library Series, 37, 141-146 (1998).

 

Ohnishi, M., Yamawaki-Kataoka, Kariya, K., Tamada, M., Hu, C.-D., and Kataoka, T. Selective inhibition of Ras interaction with its particular effector by synthetic peptides corresponding to the Ras effector region, J. Biol. Chem., 273, 10210-10215 (1998).

 

Shirouzu, M., Morinaka, K., Koyama, S., Hu, C.-D., Hori-Tamura, N., Okada, T., Kariya, K., Kataoka, T., Kikuchi, A, and  Yokoyama, S. Interactions of the amino acid residue at position 31 of the c-Ha-Ras with Raf-1 and RalGDS, J. Biol. Chem., 273, 7737-7742 (1998).

 

Shibatohge, M., Kariya, K., Liao, Y., Hu, C.-D., Watari, Y., Goshima, M., Shima, F., and Kataoka, T. Identification of PLC210, a C. elegans homolog of phospholipase C, as a putative effector of Ras, J. Biol. Chem., 273, 6218-6222 (1998).

 

Tamada, M., Hu, C.-D., Kariya, K., Okada, T., and Kataoka, T. Membrane recruitment of Raf-1 by association is not only the major function of Ras in Raf-1 activation, Oncogene, 15, 2959-2964 (1997).  

 

Hu, C.-D., Kariya, K., Kotani, G., Shirouzu, M., Yokoyama, S., and Kataoka, T. Coassociation of Rap1A and Ha-Ras with Raf-1 N-terminal region interferes with Ras-dependent activation of Raf-1. J. Biol. Chem., 272, 11702-11705 (1997).

 

Yanagihara, C., Shinkai, M., Kariya, K., Yamawaki-Kataoka, Y., Hu, C.-D., Masuda, T., and Kataoka, T. Association of elongation factor 1a and ribosomal protein L3 with the proline-rich region of yeast adenylyl cyclase-associated protein CAP. Biochem. Biophys. Res. Commun., 232, 503-507(1997).

 

Hu, C.-D., Kariya, K., Tamada, M., Akasaka, K., Shirouzu, M., Yokoyama, S., and Kataoka, T.  Cysteine-rich region of Raf-1 interacts with activator domain of post-translationally modified Ha-Ras. J. Biol. Chem., 270, 30274-30277 (1995).

 

Hu, C.-D., Zhan, Z.-L. and He, S.-P. Study on the influential factors and the sensitivity of microtitre fluctuation test. Journal of Healthy and Toxicology, 4, 115-118 (1990) (in Chinese).

 

Hu, C.-D., Zhan, Z.-L., and He, S.-P. Study on the mutagenicity of trichloromethane. Chinese Journal of Public Health, 5, 220-222 (1990) (in Chinese).

 

Hu, C.-D. and Zhang, X.-H. Influence of EM on spleen cells NK activity and its mechanisms. Chinese Journal  of Microbiology and Immunology, 8, 11-14 (1989) (in Chinese).

 

Hu, C.-D. and Zhang, X.-H. Influence of EM on specific immune responses in normal Swiss mice. Chinese Journal of Immunology, 4, 176-178 (1988) (in Chinese).

 

Hu, C.-D., Zhang, X.-H., and Bi, E.-H. Role of macrophages in the modulation of NK    activity. Foreign Medicine, Part of Immunology, 10, 16-20 (1987) (review in Chinese).